Regulatory

Part:BBa_K3142012:Design

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-10-13)


Glucose starvation promoter（PT-αcrp）


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 26
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 26
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 26
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 26
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for lactic acid bacteria.

Source

References

[1] Busby, S., and Ebright, R. H. (1999) Transcription Activation by Catabolite Activator 519 Protein (CAP). J. Mol. Biol. 293, 199–213. 520

[2] Savery, N. J., Lloyd, G. S., Kainz, M., Gaal, T., Ross, W., Ebright, R. H., Gourse, R. L., and 521 Busby, S. J. W. (1998) Transcription activation at Class II CRP-dependent promoters: identification of 522 determinants in the C-terminal domain of the RNA polymerase α subunit. EMBO J. 17, 3439–344

[3] Lloyd, G. S., Busby, S. J. W., and Savery, N. J. (1998) Spacing Requirements for 529 interactions between the C-terminal domain of α subunit of Escherichia coli RNA polymerase and the 530 cAMP receptor protein. Biochem. J. 420, 413–420.